Isolation and Modification of Ethyl-p-Methoxycinnamate from Kaempferia galanga and Its Antioxidant Activity

Vidia Afina Nuraini, Farah Azizah Hanan, Iqbal Mustapha

Sari


Oxidative stress has been associated with the development of many diseases. Kaempferia galanga, also known as aromatic ginger, is widely distributed in Asian countries, including Indonesia, and has been traditionally used as an herbal medicine to treat various ailments. Its main constituent, ethyl-p-methoxycinnamate (EPMC), has been reported to exhibit various biological activities, including anticancer, anti-inflammatory, and antituberculosis. However, there has been little research on the antioxidant activity of EPMC. Therefore, this study aimed to investigate the antioxidant properties of EPMC isolated from the rhizome of K. galanga and its hydrolyzed compound. EPMC was successfully isolated from the n-hexane extract of K. galanga in a 5.88% yield. Hydrolysis of EPMC under basic conditions resulted in p-methoxycinnamic acid (PMCA) in a 21% yield. Results of in vitro antioxidant assay showed that PMCA gave stronger scavenging activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals with an IC50 value of 518.58 ppm compared to EPMC (IC50 >1000 ppm). These results featured the importance of the hydroxyl group in enhancing the antioxidant properties of EPMC.


Kata Kunci


Antioxidant activity; ethyl-p-methoxycinnamate; hydrolysis; Kaempferia galanga; p-methoxycinnamic acid

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Referensi


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DOI: https://doi.org/10.17509/ci.v4i1.69178

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